rabbit anti cald1  (Proteintech)

Journal: Translational Cancer Research

Article Title: CALD1 inhibits invasion of human ovarian cancer cells by affecting cytoskeletal structure and the number of focal adhesion

doi: 10.21037/tcr-24-1375

Figure Lengend Snippet: CALD1 shRNA primer design

Article Snippet: Subsequently, the slides were incubated with rabbit anti-human CALD1 antibody (1:2,000; Cat No. AF6411; Affinity, Jiangsu, China) for 1 hour at 37 °C, followed by goat anti-rabbit Horseradish Peroxidase (HRP) (1:5,000; Cat No. SA00001-1; Proteintech, Wuhan, China) for 1 hour.

Techniques: shRNA, Sequencing

Journal: Translational Cancer Research

Article Title: CALD1 inhibits invasion of human ovarian cancer cells by affecting cytoskeletal structure and the number of focal adhesion

doi: 10.21037/tcr-24-1375

Figure Lengend Snippet: Differential analysis of CALD1 expression in tumor tissue and healthy tissue. (A) Analysis of CALD1 expression levels in ovarian cancer and normal tissues from TCGA and GTEx databases. (B) Comparison of the differences in CALD1 expression between ovarian cancer tissues and normal tissues on tissue microarrays. (C,D) Representative image of immunohistochemistry of normal tissue on tissue microarray. (E,F) Representative image of immunohistochemistry of ovarian cancer tissue on tissue microarray. Scale bar: 200 µm. ****, P<0.0001. TPM, transcripts per million; OV, ovarian cancer; H-Score, Histochemistry Score; TCGA, The Cancer Genome Atlas; GTEx, Genotype-Tissue Expression.

Article Snippet: Subsequently, the slides were incubated with rabbit anti-human CALD1 antibody (1:2,000; Cat No. AF6411; Affinity, Jiangsu, China) for 1 hour at 37 °C, followed by goat anti-rabbit Horseradish Peroxidase (HRP) (1:5,000; Cat No. SA00001-1; Proteintech, Wuhan, China) for 1 hour.

Techniques: Expressing, Comparison, Immunohistochemistry, Microarray

Journal: Translational Cancer Research

Article Title: CALD1 inhibits invasion of human ovarian cancer cells by affecting cytoskeletal structure and the number of focal adhesion

doi: 10.21037/tcr-24-1375

Figure Lengend Snippet: Drug sensitivity analysis of high and low CALD1 expression groups. This figure presents a drug sensitivity analysis for groups with high and low CALD1 expression, performed using the ‘oncopredict’ R package. Each boxplot illustrates the sensitivity differences for a specific drug between the high expression group (red) and the low expression group (blue). The y-axis represents drug sensitivity, while the x-axis categorizes the groups based on CALD1 expression levels. The P values above each boxplot indicate the statistical significance of the differences observed between the two groups. Drugs analyzed include irinotecan, oxaliplatin, topotecan, CDK9_5038, entospletinib, AZD5363, AZD5991, LCL161, GNE-317, tozasertib, entinostat, and telomerase inhibitor IX.

Article Snippet: Subsequently, the slides were incubated with rabbit anti-human CALD1 antibody (1:2,000; Cat No. AF6411; Affinity, Jiangsu, China) for 1 hour at 37 °C, followed by goat anti-rabbit Horseradish Peroxidase (HRP) (1:5,000; Cat No. SA00001-1; Proteintech, Wuhan, China) for 1 hour.

Techniques: Expressing

Journal: Translational Cancer Research

Article Title: CALD1 inhibits invasion of human ovarian cancer cells by affecting cytoskeletal structure and the number of focal adhesion

doi: 10.21037/tcr-24-1375

Figure Lengend Snippet: Construction and enrichment analysis of CALD1 gene network. (A) Construction of CALD1 gene network using GeneMANIA. If two genes have a relationship, such as co-expression, shared protein domain, physical interactions, co-localization, or pathway, connecting lines are drawn. The thickness of the lines represents the degree to which two genes are similar. (B,C) GO enrichment analysis and KEGG enrichment analysis. Bar chart of GO and KEGG enrichment analysis results sorted by P value. GO, Gene Ontology; GeneMANIA, Gene Multiple Association Network Integration Algorithm; KEGG, Kyoto Encyclopedia of Genes and Genomes.

Article Snippet: Subsequently, the slides were incubated with rabbit anti-human CALD1 antibody (1:2,000; Cat No. AF6411; Affinity, Jiangsu, China) for 1 hour at 37 °C, followed by goat anti-rabbit Horseradish Peroxidase (HRP) (1:5,000; Cat No. SA00001-1; Proteintech, Wuhan, China) for 1 hour.

Techniques: Expressing

Journal: Translational Cancer Research

Article Title: CALD1 inhibits invasion of human ovarian cancer cells by affecting cytoskeletal structure and the number of focal adhesion

doi: 10.21037/tcr-24-1375

Figure Lengend Snippet: Determination of lentivirus titers

Article Snippet: Subsequently, the slides were incubated with rabbit anti-human CALD1 antibody (1:2,000; Cat No. AF6411; Affinity, Jiangsu, China) for 1 hour at 37 °C, followed by goat anti-rabbit Horseradish Peroxidase (HRP) (1:5,000; Cat No. SA00001-1; Proteintech, Wuhan, China) for 1 hour.

Techniques:

Journal: Translational Cancer Research

Article Title: CALD1 inhibits invasion of human ovarian cancer cells by affecting cytoskeletal structure and the number of focal adhesion

doi: 10.21037/tcr-24-1375

Figure Lengend Snippet: Screening and construction of cell lines with stable knockdown of CALD1 . (A) Fluorescence photomicrographs of SK-OV-3 cells 72 h after lentivirus infection. (B,C) Knockdown of CALD1 in SK-OV-3 cell lines analyzed by qRT-PCR and WB. One-way ANOVA was used for statistical analysis. *, P<0.05; **, P<0.01. NC, negative control group; qRT-PCR, quantitative real-time polymerase chain reaction; WB, western blot; ANOVA, analysis of variance.

Article Snippet: Subsequently, the slides were incubated with rabbit anti-human CALD1 antibody (1:2,000; Cat No. AF6411; Affinity, Jiangsu, China) for 1 hour at 37 °C, followed by goat anti-rabbit Horseradish Peroxidase (HRP) (1:5,000; Cat No. SA00001-1; Proteintech, Wuhan, China) for 1 hour.

Techniques: Knockdown, Fluorescence, Infection, Quantitative RT-PCR, Negative Control, Real-time Polymerase Chain Reaction, Western Blot

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Subcellular Distribution and Relative Expression of Fibrocyte Markers in the CD/1 Mouse Cochlea Assessed by Semiquantitative Immunogold Electron Microscopy

doi: 10.1369/0022155411421801

Figure Lengend Snippet: Transmitted light (A) and immunofluorescence (B–G) microscopy of wax sections of spiral ligament in a midmodiolar plane, labeled with each of the antibodies and viewed by confocal microscopy. (A) The locations of the different fibrocyte types (I–V), the Reissner membrane (RM), and stria vascularis (sv) are shown. This section is the same as the one shown in B. (B) Caldesmon is localized along the side of the ligament adjacent to the bony wall, the location of the type III cells. (C) S-100 is localized throughout the ligament but appears to be absent from a thin line, probably representing the type III cell region (*). (D) AQP1 is localized along the margin of the ligament, where type III cells occur. The difference in appearance of the labeling compared with caldesmon, which localizes to the same region, is probably due to the fact that the AQP1 is a membrane protein while caldesmon is cytoplasmic. The former probably therefore outlines the cells rather than filling them. (E) Na,K-ATPase is localized to type II and type V regions (arrows) and is also strongly expressed in the stria vascularis (sv) but more weakly elsewhere. (F) CK-BB is localized to type II, III, IV, and V regions. (G) CTGF is localized to type II, III, and IV regions. Scale bar = 50 µm.

Article Snippet: Caldesmon , Acris Antibodies GmbH, Herford, Germany , S 783 , 1:10 (S).

Techniques: Immunofluorescence, Microscopy, Labeling, Confocal Microscopy, Membrane

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Subcellular Distribution and Relative Expression of Fibrocyte Markers in the CD/1 Mouse Cochlea Assessed by Semiquantitative Immunogold Electron Microscopy

doi: 10.1369/0022155411421801

Figure Lengend Snippet: Immunogold labeling of type I cells. (A) Labeling for caldesmon (CALD) is sparse and primarily cytoplasmic (arrows). (B) Labeling for AQP1 is practically absent. (C) Labeling for S-100 is strong in the cytoplasm and stronger in the nucleus (n), while mitochondria and chromatin tend to have relatively few gold particles over them in comparison. (D) Labeling for α1Na,K-ATPase is weak but present on the plasma membrane and less commonly in the cytoplasm. It is enriched over the few fine processes that extend from these cells (inset). Scale bars: A, D, and D inset = 1 µm; B and C = 2 µm.

Article Snippet: Caldesmon , Acris Antibodies GmbH, Herford, Germany , S 783 , 1:10 (S).

Techniques: Labeling, Comparison, Clinical Proteomics, Membrane

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Subcellular Distribution and Relative Expression of Fibrocyte Markers in the CD/1 Mouse Cochlea Assessed by Semiquantitative Immunogold Electron Microscopy

doi: 10.1369/0022155411421801

Figure Lengend Snippet: Immunogold labeling of type II cells. (A) Labeling for caldesmon (CALD) is sparse and both cytoplasmic (arrows) and nuclear (arrowheads). (B) Labeling for AQP1 is also sparse but present on the plasma membrane of the fine processes (arrows) and occasionally in the cytoplasm (arrowhead). (C) Labeling for S-100 is strong in the cytoplasm and stronger in the nucleus (n), while mitochondria (arrows) and chromatin have relatively few gold particles over them. Labeling is also present in the fine processes. (D) Labeling for α1Na,K-ATPase is moderate and specific mostly to the plasma membrane, especially the fine processes, and less commonly in the cytoplasm. Scale bars: A–C = 2 µm; D = 1 µm.

Article Snippet: Caldesmon , Acris Antibodies GmbH, Herford, Germany , S 783 , 1:10 (S).

Techniques: Labeling, Clinical Proteomics, Membrane

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Subcellular Distribution and Relative Expression of Fibrocyte Markers in the CD/1 Mouse Cochlea Assessed by Semiquantitative Immunogold Electron Microscopy

doi: 10.1369/0022155411421801

Figure Lengend Snippet: Immunogold labeling of type III cells. (A) Labeling for caldesmon (CALD) is moderate and present in both the cytoplasm and nucleus. (B) Labeling for AQP1 is also moderate but present primarily on membranes of the cell body and processes (inset) and occasionally in the cytoplasm (arrowhead). (C) Labeling for S-100 is weak in the cytoplasm and slightly stronger in the nucleus. (D) Labeling for α1Na,K-ATPase is weak to moderate and mostly in the cytoplasm, although with some plasma membrane labeling. Scale bars: A–C = 2 µm; B inset and D = 1 µm.

Article Snippet: Caldesmon , Acris Antibodies GmbH, Herford, Germany , S 783 , 1:10 (S).

Techniques: Labeling, Clinical Proteomics, Membrane

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Subcellular Distribution and Relative Expression of Fibrocyte Markers in the CD/1 Mouse Cochlea Assessed by Semiquantitative Immunogold Electron Microscopy

doi: 10.1369/0022155411421801

Figure Lengend Snippet: Immunogold labeling of type IV cells. (A) Labeling for caldesmon (CALD) is weak and present in both the cytoplasm (arrows) and nucleus (arrowheads). (B) Labeling for AQP1 is virtually absent. (C) Labeling for S-100 is moderate to strong in the cytoplasm and slightly stronger in the nucleus. (D) Labeling for α1Na,K-ATPase is weak to moderate and in the cytoplasm (white arrows), nucleus (white arrowheads), and on the plasma membrane (black arrows). Scale bars = 2 µm.

Article Snippet: Caldesmon , Acris Antibodies GmbH, Herford, Germany , S 783 , 1:10 (S).

Techniques: Labeling, Clinical Proteomics, Membrane

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Subcellular Distribution and Relative Expression of Fibrocyte Markers in the CD/1 Mouse Cochlea Assessed by Semiquantitative Immunogold Electron Microscopy

doi: 10.1369/0022155411421801

Figure Lengend Snippet: Immunogold labeling of type V cells. (A) Labeling for caldesmon (CALD) is weak and present mainly in the cytoplasm (arrows). (B) Labeling for AQP1 is weak to moderate and primarily on the membranes of fine processes near the cell body (arrowheads) and wider processes that extend into the scala vestibuli (sv) (inset). (C) Labeling for S-100 is moderate to strong in the cytoplasm of the cell body and fine processes, sparing the mitochondria (inset). (D) Labeling for α1Na,K-ATPase is moderate and primarily on the plasma membrane in both fine processes and the wider processes extending into scala vestibuli (sv) (inset). Scale bars: A–C = 2 µm; B inset, D, and D inset = 1 µm; C inset = 0.25 µm.

Article Snippet: Caldesmon , Acris Antibodies GmbH, Herford, Germany , S 783 , 1:10 (S).

Techniques: Labeling, Clinical Proteomics, Membrane

Journal: Journal of Histochemistry and Cytochemistry

Article Title: Subcellular Distribution and Relative Expression of Fibrocyte Markers in the CD/1 Mouse Cochlea Assessed by Semiquantitative Immunogold Electron Microscopy

doi: 10.1369/0022155411421801

Figure Lengend Snippet: Histograms showing the relative labeling density in fibrocytes from each sample (left) and the mean across all four samples (right) for caldesmon (above line) (samples 1B, 2B, 3B, and 5A), AQP1, S-100, and α1Na,K-ATPase (below line) (samples Hide, 4109, 4178, and 4179). Caldesmon and AQP1 are most highly expressed in type III fibrocytes, with the latter to a greater extent. S-100 is expressed to a similar extent in types I, II, and V and is only weakly present in types III and IV. α1Na,K-ATPase is expressed most strongly and to a similar degree in type II and type V fibrocytes and more weakly in types I, III, and IV. Bars = SEM.

Article Snippet: Caldesmon , Acris Antibodies GmbH, Herford, Germany , S 783 , 1:10 (S).

Techniques: Labeling